Effect of Ultrasonic Amplitude on the Yield and Properties of Barramundi (Lates calcarifer) Skin Collagen

Barramundi skin, a by-product of the fish processing industry, has shown potential as an alternative collagen source. However, commonly used acid extraction method to produce rendered low yield requires lengthy time and is not environmentally friendly. As result, adoption greener technology, such ultrasound, improve conventional process emerging. This study aimed investigate effect different ultrasonication amplitudes on recovery from barramundi skin. The resulting collagens were evaluated for their protein, hydroxyproline moisture content, colour, molecular weight distribution, FTIR spectra. Ultrasound-assisted (UAE) was performed at 40 (UAE40), 60 (UAE60) 80 (UAE80) % amplitude 20 min. For comparison, acetic also carried out acid-soluble (ASC). UAE increased (p<0.05) with UAE80 exhibiting 7-fold increment compared ASC. Increasing ultrasonic considerably but decreased indicating reduction in quality. Furthermore, protein content SDS-PAGE profile extracted revealed that promoted degradation. spectra indicated despite slightly varying wavenumbers, no detrimental triple helical structure seen following presence amides A, B, I, II, III. Also, α1, α2 ß-chains found all samples, although band intensity reduced increased. In conclusion, given right conditions, could without influencing structure.